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SM 9000光譜儀

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SM9000光譜儀是一種高分辨率光纖光譜測量儀,測量范圍涵蓋紫外光、可見光乃至近紅外波段。SM9000既可以單獨使用,也可以與FKM多光譜熒光動態顯微成像系統、FL3500雙調制葉綠素熒光儀等儀器聯用,測量各種熒光的光譜組成。由于其具備超高的靈敏度,甚至可以測量單個細胞激發熒光的光譜。每秒可記錄100組16bits分辨率的光譜數據。

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功能特點:

·超高靈敏度,可檢測單個細胞的熒光光譜

·超高分辨率,可檢測10μs - 10ms的閃光

·采集頻率達100次/秒,可檢測動態光譜

·積分時間從1毫秒到數分鐘可調

·模塊化設計,小巧耐用,熱穩定性高

·產熱量極低

技術參數:

·光譜范圍:200 - 980nm

·分辨率:可檢測10μs - 10ms的閃光

·采集頻率:100次/秒

·積分時間:1毫秒到數分鐘可調

·光學入口:直徑0.5,數值孔徑(NA)=0.22,可拆卸SMA接頭

·入射狹縫:70µm×1400µm

·光柵:平場型校正

·波長精確度:< 0.5nm

·再現性:< 0.1nm

·溫度漂移:< 0.01nm/K

·像素光譜距離:0.8nm

·FWHM半高寬:3 - 4nm

·雜散光:0.1%(氙燈340nm測量)

·CCD陣列像素數:1044×64

·像素尺寸:24×24mm2

·系統數據:16Bit模數轉換

·可聯用儀器:FKM多光譜熒光動態顯微成像系統、FL3500雙調制葉綠素熒光儀等

與FKM多光譜熒光動態顯微成像系統聯用的SM9000

應用案例:

FKM系統聯用研究銅指示植物海州香薷Elsholtzia splendens的葉綠素熒光及其光譜組成(Peng2013Environ. Sci. Technol


與FL3500系統聯用研究藍隱藻Guillardia theta的葉綠素熒光及其光譜組成(Cheregi,2015,Journal of Experimental Botany)

產地:歐洲

參考文獻:

1. Bernát G, et al. 2017. On the origin of the slow M–T chlorophyll a luorescence decline in cyanobacteria: interplay of short-term light-responses. Photosynthesis Research, DOI 10.1007/s11120-017-0458-8

2. Selyanin V, et al. 2016. The variability of light-harvesting complexes in aerobic anoxygenic phototrophs. Photosynthesis research, 128(1): 35-43

3. Tilstone G, et al. 2016. Effect of CO2 enrichment on phytoplankton photosynthesis in the North Atlantic sub-tropical gyre. Progress in Oceanography, 158: 76-89

4. Mishra K B, et al. 2016. Plant phenotyping: a perspective. Indian Journal of Plant Physiology, 21(4): 514-527

5. Cheregi O, Kotabová E, Prá?il O, et al. 2015. Presence of state transitions in the cryptophyte alga Guillardia theta . Journal of Experimental Botany, 66: 6461-6470

6. Li G, Brown C M, Jeans J A, et al. 2015. The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytologist, 205:533-543

7. Kotabová E, Jare?ová J, Kaňa R, et al. 2014. Novel type of red-shifted chlorophyll a antenna complex from Chromera velia. I. Physiological relevance and functional connection to photosystems. Biochimica et Biophysica Acta – Bioenergetics, 1837:734-743

8. ?ebela D, Olejní?ková J, Sotolá? R, et al. 2014. The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition. BBA , 1817: 1237-1247

9. Peng H, et al. 2013. Toxicity and De?ciency of Copper in Elsholtzia splendens A?ect Photosynthesis Biophysics, Pigments and Metal Accumulation. Environ. Sci. Technol., 47 (12): 6120-6128



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